Phosphop53 ser15 antibody 9284s from cell signaling. We have, therefore, generated a panel of antizebrafish p53 monoclonal antibodies and. Puma, a novel proapoptotic gene, is induced by p53. Applications key ab array antibody array ae affinity electrophoresis affin chroma. Antibodyplus provides trial size antibody samples for antibody validation. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases. Deltamethrin is responsible for health and reproduction problems both in mammals and aquatic organisms. Detection of the p53 response in zebrafish embryos using new. Please use this form to recommend updates to the information in zfin. Genetic modeling of lifraumeni syndrome in zebrafish. Recombinant full length protein corresponding to zebrafish p53. P53 is a reported alias name for the human gene tp53, or tumor protein p53.
However, any fluorophoreconjugated antibody with a userpreferable absorptionemission spectra can be used here. In response to cytokines and growth factors, stat family members are phosphorylated by the receptor. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish. Although p53 plays a critical role in cell cycle control and the regulation of apoptosis, it is largely dispensable for normal embryonic development in fish and mice. Furthermore, as with some lfs mutations, the zebrafish p53i166t allele is a. Recent developments in p53 antibody research p53 is a stressactivated transcription factor, encoded by the tp53 gene.
The completion of the human genome sequence international human genome sequence consortium 2001nature 409, 860921. In this system the p53 gene is identified as a p53responsive gene itself. Chk 2 and chk 1 c an phos phory lat e p53 at s er20, enhanc ing it s t et rameriz at ion, s t abilit y. It is aimed to help labome visitors find the most suited p53 antibody.
Analyses of p53 target genes in the human genome by. Zebrafish models of p53 functions pubmed central pmc. Hrpcoupled antimouse and antirabbit antibodies dako, glostrup, denmark. In this book, sue armstrong wishes to inform you about p53, a key tumorsuppressor gene or more grandly named as the the guardian of the genome, by telling you stories of the scientists who tried to understand it. Replacement to abcam, santa cruz, sigma and cst antibody. Furthermore, compared to wildtype siblings, heterozygous stac fish develop invasive tumors at a dramatically reduced rate, suggesting a reduced cancer risk. Anti p53 antibody a 1 is recommended for detection of p53 of mouse, rat and human origin by wb, ip, if and elisa.
In response to cytokines and growth factors, stat family members are phosphorylated by the receptor associated kinases, and then form homo or heterodimers that translocate to the cell nucleus where they act as transcription activators. Antip53 antibody products listed below are antip53 antibodies from multiple suppliers. Our results demonstrate that zebrafish p53 protein can readily be induced in embryos and detected using these new antibody tools, which will increase the usefulness of zebrafish as a model in. Such mutations probably facilitate carcinogenesis primarily through abrogating the tumor suppressor activities of the wild type p53 protein, although. The gene that cracked the cancer code by sue armstrong. Genetic modeling of lifraumeni syndrome in zebrafish disease. P hos phory lat ion of p53 at s er46 regulat es t he abilit y of p53 t o induc e apopt os is 16. Zebrafish have a competent p53dependent nucleotide. P53 antibody rabbit polyclonal from proteintech validated in western blot wb, immunoprecipitation ip, immunofluorescence if, coimmunoprecipitation ip, chimmunoprecipitation ip, enzymelinked. Detection of phosphop53 and p53 detection in ncih460 cells post hydroxyurea treatment hydroxyurea is an inducer of p53 upregulation and phosphorylation as hydroxyurea concentration. Introduction p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. Activation of p53 can lead to either cell cycle arrest and dna repair or.
Authors conclude that quantitative measurements of intratumor heterogeneity by multiplex fish. Mutational inactivation of the p53 gene product is one of the most common genetic changes seen in human cancers. In this study, zebrafish adults were exposed for 15 days to 0. P53 antibody rabbit polyclonal from proteintech validated in western blot wb, immunoprecipitation ip, immunofluorescence if, coimmunoprecipitation ip, chimmunoprecipitation ip, enzymelinked immunosorbent assay elisa applications. The zebrafish information network zfin is the database of genetic and genomic data for the zebrafish danio rerio as a model organism.
We appreciate as much detail as possible and references as appropriate. Through the personal accounts of key researchers, the book reveals the excitement of the hunt for new curesthe hype, the enthusiasm, the lost opportunities, the blind alleys and the thrilling breakthroughs. Pdf detection of the p53 response in zebrafish embryos. A c et y lat ion of p53 is mediat ed by p300 and cb p ac et y lt rans f eras es. Genetic screen for lifraumeni syndrome in zebrafish. Tumor protein p53, also known as p53, cellular tumor antigen p53 uniprot name, the guardian of the genome, phosphoprotein p53, tumor suppressor p53, antigen nyco, or transformationrelated. Loss of p53 response is associated with cell proliferation and invasion, and mutations in the p53 gene are found in over 50% of all human cancers. Detection of phosphop53 and p53 detection in ncih460 cells post hydroxyurea treatment hydroxyurea is an inducer of p53 upregulation and phosphorylation as hydroxyurea concentration increases, we observe an increase in p53 and phosphop53 signal, and this is depicted in both the image and graphical data above. We investigated the chronic effects of deltamethrin on gonads by histopathological examination. Detection of the p53 response in zebrafish embryos using.
Chk 2 and chk 1 c an phos phory lat e p53 at s er20, enhanc ing it s t et rameriz at ion, s t abilit y, and ac t iv it y 8, 9. Antimutant p53 antibody y5 bsa and azide free ab219731 specific references 6 description. Analysis of apoptosis in zebrafish embryos by wholemount. Delay in development and behavioural abnormalities in the absence.
From abcams oem supplier st johns laboratory, validated in wb, elisa. Zebrafish p53 is highly similar to mammalian p53 in both structure and function. Jmse free fulltext toxicity of deltamethrin to zebrafish. Rabbit polyclonal antip53 mono methyl lys370 antibody stj97694. A guide for the laboratory use of zebrafish danio rerio.
Our results demonstrate that zebrafish p53 protein can readily be induced in embryos and detected using these new antibody. The hrpconjugated antirabbit igg antibody gtx211001 was used to detect the primary antibody. This antibody reacts with human, rat, mouse samples. Smc3 knockdown triggers genomic instability and p53dependent apoptosis in human and zebrafish cells. A 53 kda band corresponding to p53 was observed across cell lines tested except skov3. Retinal precursor cells mutant for caf1b arrest in s phase and undergo p53 mediated apoptosis. Through the personal accounts of key researchers, the book reveals the excitement of the hunt for new curesthe hype, the enthusiasm, the. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa.
Chromatin immunoprecipitations were performed with crosslinked chromatin from hct116 cells. Chromatin immunoprecipitations were performed with crosslinked chromatin from hct116 cells treated with uv 100 jm 2 followed by a 3 hour recovery and either p53 1c12 mouse mab or normal rabbit. Furthermore, compared to wildtype siblings, heterozygous stac fish develop invasive tumors at a. Mitotic cells in stac mutants show chromosome missegregation and are usually arrested in g2m phase. Immunoprecipitation and western blot of p53 protein in hct116 lysates treated with 30um cisplatin, 24 hours using a input, 20 g of hct116 lysates b normal rabbit igg or c 2. Tumor suppressor protein p53 functions as a transcription factor regulating genes involved in cellular responses to stress. Western blot analysis of cell extracts using p53 antibody. After uv dna damage, the tumor suppressor p53 plays a critical role in halting the cell cycle to allow for repair of the damage, or initiating cell death. Loss of p53 synthesis in zebrafish tumors with ribosomal protein. The protein encoded by this gene is a member of the stat family of transcription factors. The availability of antibodies against zebrafish p53 protein would, therefore, offer a prospect of a powerful system for identification of novel. Tp53 antibody mouse monoclonal p53 antibody prospec.
An important tumor suppressor, the protein mediates cellular growth and proliferation. In addition to differences in development, p53null zebrafish embryos also showed. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Oct, 2019 results demonstrate that zebrafish p53 protein can readily be induced in embryos and detected using these new antibody tools, which will increase the usefulness of zebrafish as a model in compoundbased screening for novel drugs in cancer research. Anti p53 antibody products listed below are anti p53 antibodies from multiple suppliers. Overexpression of wildtype p53 protein in p53 deficient cells can arrest cell proliferation, reverse a tumorigenic phenotype, and sometimes induce apoptosis or differentiation 1, 2. For example, the fulllength amino acid sequence of zebrafish p53 is 48. Rabbit monoclonal y5 to mutant p53 bsa and azide free. Activation of p53 leads to induction of dna repair, cellcycle arrest, apoptosis and senescence. It is not a book that will tell you the detailed molecular mechanism by which this gene will prevent or cause cancer. An important tumor suppressor, the protein mediates cellular growth and proliferation, regulating proteins involved in the stressresponse. Antibody ab32049 antimutant p53 antibody y5 by abcam. The data collected includes not only links to publications in. Activation of p53 leads to induction of dna repair, cellcycle arrest, apoptosis and.
Nov 20, 2014 in this book, sue armstrong wishes to inform you about p53, a key tumorsuppressor gene or more grandly named as the the guardian of the genome, by telling you stories of the scientists who tried to understand it. Tumor protein p53, also known as p53, cellular tumor antigen p53 uniprot name, the guardian of the genome, phosphoprotein p53, tumor suppressor p53, antigen nyco, or transformationrelated protein 53 trp53, is any isoform of a protein encoded by homologous genes in various organisms, such as tp53 humans and trp53 mice. Not surprisingly, p53 is the most studied single gene in history. The p53 promoter polymorphism is not associated with the susceptibility of uterine leiomyomas in iranian women. Zfin provides a wide array of expertly curated, organized and cross. This antibody gave a positive signal in roscovitinetreated zebrafish embryo lysates. Zebrafish antibodies primary antibodies sigmaaldrich. Spliceacceptor site mutation in p53 gene of hu888 zebrafish line. Due to their striking genomic similarity to humans, regenerative abilities. Fulllength zebrafish p53 is 48% identical in amino acid sequence to human p53 cheng et al. Zfin provides a wide array of expertly curated, organized and crossreferenced zebrafish research data. I nhibit ion of deac et y lat ion s uppres s ing mdm2 f rom rec ruit ing hda c1 c omplex by p19 a rf s t abiliz es p53. Based on the observation made above, we designed an f3 recessive genetic screen in zebrafish to identify components of the lfsp53. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development.
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